ABYSS

ABYSS(1)                         User Commands                        ABYSS(1)



NAME
       ABYSS - assemble short reads into contigs

SYNOPSIS
       ABYSS [OPTION]... FILE...

DESCRIPTION
       Assemble all input files, FILE, which may be in FASTA, FASTQ, qseq,
       export, SRA, SAM or BAM format and may be compressed with gz, bz2 or xz
       and may be tarred.

       Users wishing to run a full assembly with ABySS are recommended to use
       abyss-pe as their entry point rather than the ABYSS program.  abyss-pe
       is a Makefile that coordinates many of the ABySS tools (including
       ABYSS) in order to run the full ABySS assembly pipeline.  abyss-pe is
       capable of doing many things that ABYSS is not, such as leveraging the
       distance information provided by paired end reads and mate pair reads.

       --chastity
              discard unchaste reads [default]

       --no-chastity
              do not discard unchaste reads

       --trim-masked
              trim masked bases from the ends of reads [default]

       --no-trim-masked
              do not trim masked bases from the ends of reads

       -q, --trim-quality=THRESHOLD
              trim bases from the ends of reads whose quality is less than the
              threshold

       -Q, --mask-quality=THRESHOLD
              mask all bases in reads whose quality is less than the threshold

       --standard-quality
              zero quality is `!' (33)
              default for FASTQ and SAM files

       --illumina-quality
              zero quality is `@' (64)
              default for qseq and export files

       -o, --out=FILE
              write the contigs to FILE

       -k, --kmer=KMER_SIZE
              k-mer size

       -t, --trim-length=TRIM_LENGTH
              maximum length of dangling edges to trim

       -c, --coverage=COVERAGE
              remove contigs with mean k-mer coverage less than this threshold

       -b, --bubbles=N
              pop bubbles shorter than N bp (default: 3*k).

       -b0, --no-bubbles
              do not pop bubbles

       -e, --erode=COVERAGE
              erode bases at the ends of blunt contigs with coverage less than
              this threshold

       -E, --erode-strand=COVERAGE
              erode bases at the ends of blunt contigs with coverage less than
              this threshold on either strand

       --coverage-hist=FILE
              record the k-mer coverage histogram in FILE

       -g, --graph=FILE
              generate a graph in dot format

       -s, --snp=FILE
              record popped bubbles in FILE

       -v, --verbose
              display verbose output

       --help display this help and exit

       --version
              output version information and exit

AUTHOR
       Written by Jared Simpson and Shaun Jackman.

REPORTING BUGS
       Report bugs to <abyss-users@bcgsc.ca>.

COPYRIGHT
       Copyright 2015 Canada's Michael Smith Genome Sciences Centre



ABYSS (ABySS) 2.1.5                2015-May                           ABYSS(1)